BLOOD GROUPING BY TUBE TECHNIQUE CELL GROUPING

	Step 1 : Set the table and prepare the record books.
	Step 2 : Check the identity of the specimen on the labels, requisition note and the record book.
	Step 3 : Prepare 5% suspension of 3 times washed red blood cells.
	Step 4 : Label 4 small tubes for Anti-A, Anti - B, Anti-AB and Anti - D.
	Step 5 : Add one drop each of the anti-sera to the marked tubes.
	Step 6 : Add one drop of the RBC suspension to each of the tubes containing anti-sera.
	Step 7 : Mix thoroughly by shaking the tubes.
	Step 8 : Incubate at room temperature (RT) for 10 minutes.
	Step 9 : Centrifuge the tubes at 1000 rpm for 1 minute, remove the tubes and observe for haemolysis in the supernatant then shake the tube to find out if there is agglutination.
	Step 10 : If there is no visible agglutination, transfer a part of the content of the tube to a microscope slide and read under a low power lens for agglutination.
	Step 11 : Record the results.
	Step 12 : Do not discard the tubes (They may be required to be checked later).

	SERUM GROUPING (REVERSE GROUPING)
	Step 1 : Set the table and prepare the record books.
	Step 2 : Mark three tubes for A-Cells, B - Cells and O - Cells.
	Step 3 : Add one drop of the serum to be tested to each of these tubes.
	Step 4 : Take one drop of "Pooled Cells" from the panel of A-cells, B - cells and O - cells and add it to the tubes prepared in step 3.
	Step 5 : Mix well by shaking the tubes.
	Step 6 : Incubate at RT for 30 minutes.
	Step 7 : Centrifuge the tubes at 1000 rpm for 1 minute, remove the tubes and observe for haemolysis in the supernatant then shake the tubes to find out if there is agglutination.
	Step 8 : If there is no visible agglutination, transfer a part of the contents of the tube to a microscope slide and read under a low power lens for agglutination.
	Step 9 : Record the results.
	Step 10 : Do not discard the tubes (They may be required to be checked later).

	PRECAUTIONS
	Never rely on your memory.
	In case you have been distracted halfway through the test and do not remember where you had left it, Run the entire test again, from the beginning.
	Always put serum in the tubes first, check for its presence in the tube before you add cell suspension to the same tube.
	Whenever possible let someone else check your results as well as record.