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|
Take
the previously selected and properly numbered blood bag. Recheck the donor
identity and the given serial number. |
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|
Uncover
the sterile needle of the collection bag and perform phlebotomy immediately
using proper aseptic technique. |
|
|
Ask
the donor to open and close his hand after giving him rubber ball or a similar
item provided for this purpose. |
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|
The
donor should be under constant observation during blood collection and should
never be left unattended. |
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|
Place
the bag on the weighing machine and mix the blood and the anticoagulant
solution periodically.
Note the weight of the collection bag (without satellite bags in case of
double or triple bags). Add 370 gms to this to determine the total weight
of the bag after blood collection. The blood bag will contain 350 ml of
blood at this weight. |
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|
Note
the time of phlebotomy on the donor form in the space provided for it. |
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Note
the weight of the collection bag to see when it reaches the weight for collecting
350 ml blood. |
|
|
Once
enough blood is collected in the bag apply an artery forcep to collecting
tube of the set. |
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Release
pressure of the B.P. instrument. |
|
|
Remove
needle from the vein, put a sterile cotton swab with alcohol at the site
and ask the donor to fold the arm at elbow to create pressure at the site
or ask him to hold the swab with his other hand and apply pressure. Do not
allow the donor to sit up or stand immediately. |
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|
Take
the bag and pilot tubes to another table. |
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|
Release
the artery forcep and allow blood from the tubing to drain into one of the
numbered pilot tubes. |
|
|
Clamp
the tubing after it has emptied, mix the blood and anticoagulant againg
by gently agitating the bag for 15 to 20 seconds. |
|
|
Open
the clamp again and allow anti - coagulated blood to run into the second
numbered pilot tube. |
|
|
Seal
the tubing at minimum four places by using the tube-sealing machine. Ensure
that the tubing contains blood in these sealed parts. Also ensure the each
sealed part of the tubing has the serial number printed by the manufacturers. |
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|
Refrigerate
the bag between 4 and 6 degrees immediately. Do NOT refrigerate if Platelet
Concentrate is to be prepared from this unit, but keep at temperature between
20 and 24 degrees (in the 'Platelet Incubator'). |
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|
Enter
the time of completion of blood collection in the space provided on the
donor form. |
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|
Ask
the donor to sit up on the donor bed and if he does not feel giddy in sitting
position lead him to the recovery bed, make him sit there and have the refreshments.
Ask him to keep his 'donor arm' folded at elbow and holding it high (at
or near shoulder level). |
|
|
Check
the phlebotomy site to ensure the there is no bleeding from the venepuncture
site. Put a medicated adhesive tape (previously cut to about 1 square centimeter). |
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|
Thank
the donor for his donation and encourage him to donate again. |
